Comparison of nucleotide sequence of mitochondrial rRNA 16S was used to design polymerase chain reaction (PCR) primers specific for C. formosanus. The diagnostic assay consists of two independent PCR ...
Primers and oligonucleotide probes ... were cultured and then tested separately by PCR for the bacterial 16S rRNA. Of the 172 clinical cases, 17 cases tested positive by PCR.
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