Each cycle of PCR has three main steps, as described in the following sections. The number of new copies of the DNA sequence of interest doubles with each three-step cycle. Thus, if the PCR ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below ... specialized thermal cyclers with fluorimeters. Multiplex PCR 3 Multiplex ...

Pipet the emulsion into the wells of a PCR plate as 10 aliquots of 50 μl. Pipet 50 μl of the aqueous phase (Step 3) into a well as a nonemulsified control. Overlay the emulsified and ...