Each cycle of PCR has three main steps, as described in the following sections. The number of new copies of the DNA sequence of interest doubles with each three-step cycle. Thus, if the PCR ...

PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below ... specialized thermal cyclers with fluorimeters. Multiplex PCR 3 Multiplex ...

If the template DNA is complex, such as mammalian cellular DNA, then we generally use 3–30 ng of template DNA per PCR (1,000–10,000 haploid genome equivalents). All steps are performed at room ...