Now, Warnecke and colleagues are challenging the idea that these space-saving proteins are not commonplace in bacteria. In a preprint uploaded to bioRxiv this January that has yet to undergo peer ...
The team's findings, published in Nature on January 8, could eventually guide the ... of vital bodily functions—can also ...
They discovered that despite relatively weak binding by an essential protein—histone variant CENP-A/Cse4—to DNA, other proteins are recruited to one specific site in the center of the chromosome ...
High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer ... To each tube add 40 μl of protein A–agarose beads and pre-clear the solution ...