Here we report on a 3.0 Å crystal structure of a ternary complex of wild-type Thermus thermophilus argonaute bound to a 5′-phosphorylated 21-nucleotide guide DNA and a 20-nucleotide target RNA ...

Argonaute proteins inhibit gene expression by binding to messenger RNA via a small nucleic-acid guide. Structures of the Argonaute complex bound to target RNA reveal snapshots of a silencing ...

The Argonaute (Ago) enzyme complex plays a critical role in DNA and RNA target cleavage for a process known as RNA silencing in prokaryotic and eukaryotic cells, making them a target for future ...

Argonaute proteins play essential roles in RNA interference methods and also as a central component of the RNAi silencing complex (RISC).

Argonaute 2 and other Argonaute proteins regulate the influence of about a third of the genes found in humans and other mammals—and ... Yet Argonautes' workings are complex and not yet entirely ...

How argonaute proteins intervene in gene regulation process Date: November 13, 2013 ... 2022 — The gene-silencing complex HUSH might be involved in complex disorders affecting the brain and neurons.

The binding of the microRNA-Argonaute complex to its mRNA target triggers its destruction. In mammals, this destruction has, until now, ...

Our group has structurally characterized and mechanistically defined events associated with processing of long double-stranded RNAs into siRNAs by the endonuclease activity of Dicer and ...

LA JOLLA, CA – May 9, 2013 – A team led by scientists at The Scripps Research Institute (TSRI) has found how to boost or inhibit a gene-silencing mechanism that normally serves as a major ...

In another step toward realizing the elusive potential of RNA drugs, scientists at the Scripps Research Institute have nailed down one of the mechanisms by which the gene-silencers reach their ...

We could go right up to the edge of saying it was Argonaute without actually saying it." Just before he accepted defeat, however, a CSHL colleague, Leemor Joshua-Tor, invited him into a darkened room ...

The exosomal Argonaute 2 (AGO2) protein forms a complex with siRNAs, ... By reducing PBP2a levels by exosome-delivered siMecA-AGO2 complex, MRSA can be converted into methicillin-sensitive bacteria.