Small-molecule protein kinase inhibitors are widely used to elucidate cellular signaling pathways ... Here we used functional assays to profile the activity of 178 commercially available kinase ...

Signal strength in this assay depends ... The optimal concentration of kinase for this assay depends on the specific activity of the enzyme under the conditions used, and this varies greatly ...

Traditional kinase activity assays have used the incorporation of ... ATP levels decrease during the reaction and the signal will be inversely related to product formation. This assay can exhibit ...

However, these assays can only measure kinase activity in vitro ... of the Jak family and look downstream for inhibition of cell signaling, stat5 phosphorylation for example, and read-outs ...

They have many regulatory functions, and assaying kinase activity is key in many ... of surfaces that allow both quantitative assays of enzyme activity and readout by mass spectrometry," Mrksich ...

The Transcreener ADP 2 Assay uses a highly selective antibody and far-red fluorescent tracer to directly detect ADP produced by any ADP-producing enzyme (i.e. kinases, ATPases, helicases ...

If we can measure kinase activity quickly and directly, then we can potentially identify many more. One problem is that purifying kinases is not an easy task. What we did in the article was improve ...

Covalent (irreversible) Bruton’s tyrosine kinase (BTK ... paradoxically hinder BTK catalytic activity yet allow effective B-cell–receptor signaling and AKT pathway activation despite exposure ...

enzyme activity studies, reporter gene assays, signal transduction, kinase and GPCR assays. The range is so wide it’s nearly impossible to list all the applications Varioskan LUX is ideal for.

These interactions play a vital role in modulating complex signaling ... activity, localization, and function of proteins, ultimately influencing various cellular activities. Disruption in these ...