The Universal Kinase Activity Kit (Catalog # EA004) provides a convenient, nonradioactive, HTS-compatible format for assaying kinase activity. The assay can be used to characterize enzyme kinetics ...

An expert panel assembled by GEN discusses how to achieve far-reaching domino effects, intervening in (and elaborating upon) lines of cell-signaling events, designing drug screens, and devising ...

The convenient assay format will make this approach an appealing substitute for radiolabel-based kinase assays, which cannot be run in crude lysates owing to high levels of background phosphorylation.

In a breakthrough study, scientists found that silencing a single overactive enzyme in the brain reversed early damage caused ...

Stanford researchers discovered that dialing down an overactive enzyme, LRRK2, can regrow lost cellular “antennae” in key ...

Elucidating kinase-substrate relationships is critical for understanding how phosphorylation affects signal transduction and regulatory cascades. Using the α catalytic subunit of protein kinase ...

The recent SLAS conference saw a number of innovative products showcased, including the Thermo Scientific Varioskan LUX, a multimode microplate reader which can be used across a range of applications.

Kinases mediate the phosphorylation of specific substrates. They have many regulatory functions, and assaying kinase activity is key in many research areas, including drug discovery. Using matrix ...

MAPK1 enzyme titration using Transcreener ADP 2 Assay with FP readout. The enzyme buffer included 20 mM Tris pH 7.5, 10 mM MgCl 2 , 1 mM DTT, and 0.01% Tween-20.