Bisulfite Conversion of Methylated Cytosine. Cytosine methylation occurs when a methyl group binds to the fifth carbon of a cytosine to form 5-methylcytosine (5mC). Researchers cannot detect 5mC with ...

However, bisulfite converted sequencing libraries show very low diversity because the bases that appear are predominantly adenine, guanine, and thymine, with a very small fraction of cytosines.