Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

CRISPR-Cas9 is effective at generating small ... However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient.

For example, the PARP1 homology-directed repair (HDR) plasmid exploits the HDR pathway that enables precise DNA repair at the location of the CRISPR/Cas9 DSB site according to a targeted repair ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Using gene editing with CRISPR-Cas9, the team has been able to study these proteins without altering ... Dec. 13, 2024 — Microorganisms produce a wide variety of natural products that can be ...

Human extracellular matrices can be edited in their composition using the CRISPR/Cas9 system, leading to materials exhibiting tailored regenerative capacities.

Genome editing with various CRISPR-Cas molecule complexes has ... The slow variant is called homology-directed repair. Researchers want to use this method of repair because it allows the precise ...

In cystic fibrosis, researchers have used CRISPR HDR-mediated knock-in to correct mutations in the CFTR gene in airway stem cells. For sickle cell disease, the FDA has approved a CRISPR/Cas9 therapy ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

A recent review published in the journal Engineering delves into the significant advancements and potential of CRISPR technologies in the field of regenerative medicine. The study, authored by ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...