Figure 1: Approaches to genetic perturbation: shRNA knockdown, Cas9 knockout ... induced by each sgRNA, indel rate, and transcriptional activation for knockout and activation screening ...

Using ZFNs, TALENs and CRISPR/Cas9, these efforts have among others led to establishment of novel methodologies that both improve the targeting and indel detection efficacy ... method for screening ...

By dumping random bits of non-homologous DNA into the cell with Cas9, they disrupted the DNA repair process and boosted knockout efficiency up to five fold in human cell lines. CRISPR-Cas9 is the ...

The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more ...

Based on this information, they successfully made CRISPR-Cas9 reagents targeting those mutations. The scientists named this new treatment CINDELA, which stands for “Cancer-specific InDel ...

Led by Aaron Gitler, Ph.D., professor of genetics, and Michael Bassik, Ph.D., assistant professor of genetics, the team applied CRISPR/Cas9 knockout (KO) screens in human cells and in primary ...

Using their adeno-associated virus CRISPR-Cas9 components, they were able to knockout the UCP1 gene that defines brown adipose and enables it to generate heat, in adult mice. They observed that ...

CRISPR/Cas9 gene-editing technology combined with next-generation sequencing has opened the door for genome-wide screens to identify novel protein functions. CRISPR/Cas9 screens require the delivery ...

CRISPR-Cas9 gene editing technology is widely used to to help study genes of interest and modify disease-associated genes. However, the system is associated with adverse effects, including ...

Here, we walk you through everything you need to know about CRISPR-Cas9, including what it is, how it works, and what we can expect from it in the future. CRISPR-Cas9 is a new technology for ...

The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more ...