Baylor College of Medicine4y
Generation of Knockout Mice using Electroporation
CRISPR/Cas9-mediated NHEJ in mouse embryos will be used to generate founder animals harboring insertion/deletion (indel) or interval deletion knockout alleles. At this time, the core can perform ...
Baylor College of Medicine4y
Generation of Knock-in Mice Using Single-Stranded Oligodeoxynucleotides (ssODNs)
CRISPR/Cas9-initiated HDR in mouse embryos will be used to generate ... b. Epitope tag and conditional KO alleles: The inserted sequence in a correctly targeted allele will create a shift in the size ...
Purdue University4y
Genome Editing
A minimum of 3 positive founders will be delivered. To produce CRISPR/cas9-mediated gene indels to generate knock-out mice or rats. The investigator will provide or purchase the CRISPR gRNA(s). TGEF ...
Business Insider7y
Mice skin turned green by scientists using CRISPR
(REUTERS) British scientists are testing cutting edge gene editing techniques in mice, by inserting a jelly fish gene that makes their skin green. The research uses CRISPR-Cas9, a gene editing ...
Frontiers10d
CRISPR/Cas9 in Cancer: Pioneering Gene Editing for Enhanced Drug Discovery
Cancer continues to be a formidable challenge globally, with traditional drug discovery methods focusing on cytotoxic agents and targeted therapies altering the landscape of cancer prognosis and ...
eLife6mon
Functional identification of soluble uric acid as an endogenous inhibitor of CD38
Soluble uric acid at physiological levels limits NAD+ degradation and excessive inflammation by directly inhibiting CD38.
AZoLifeSciences on MSN4mon
Applications of Gene Knockout and Knock-in Models in Disease Research
Both gene knockout and knock-in models are powerful ... protein 9 systems are common gene-editing technologies, with ...