Winz et al. describe a protocol for NAD captureSeq, a method to capture and identify NAD-capped RNA sequences. Here we describe a protocol for NAD captureSeq that allows for the identification of ...

Conventional methods for preparing small-RNA–seq libraries by adaptor ligation generate a significant amount of adaptor dimer, thereby resulting in wasted sequencing reads. These methods also do ...

Circulating small RNAs in biofluids can serve as diagnostic and prognostic biomarkers for various diseases, such as cancer, cardiovascular and neurological disorders, and others. Biofluid samples ...

Next-generation sequencing has moved beyond the confines of genomic samples obtained from heterogeneous tissues and begun to disseminate information at the single-cell level. The information ...

According to Chen, PANDORA-seq uncovers a surprising small-RNA landscape that is dominated by tsRNAs and rRNA-derived small RNAs, or rsRNAs, rather than microRNAs, ...

Biomedical scientists have developed a new RNA-sequencing method -- 'Panoramic RNA Display by Overcoming RNA Modification Aborted Sequencing,' or PANDORA-seq -- that can help discover numerous ...

RNA sequencing has emerged as a powerful supplement to DNA sequencing for Mendelian disease diagnosis, but clinical translation of diagnostic RNA-seq has not been widely achieved. Researchers at ...

Single-cell RNA sequencing, or "scRNA-seq" for short, is a technique that allows scientists to study the expression of genes in an individual cell within a mixed population -- which is virtually ...

Degradome sequencing, also known as parallel analysis of RNA ends (PARE) sequencing, is a cutting-edge technique that leverages high-throughput sequencing and bioinformatics to investigate the ...

In a new paper published in the journal Nature Methods, researchers at UC Santa Cruz introduce the first-ever method for analyzing RNA sequencing data genome-wide using a “pantranscriptome,” which ...

A team led by a biomedical scientist at the University of California, Riverside, has developed a new RNA-sequencing method— "Panoramic RNA Display by Overcoming RNA Modification Aborted ...