Keep the staining reactions at as low a volume as possible to conserve reagents. For instance, you can add 25ml of a 2´ stock of stain to 25 ml of a 2´ stock of cells for a total volume of 50ml. All ...

Users must contact the Flow Cytometry and Single Cell Core Facility (FCSC) at This email address is ... 100 GB of data/slide) depending on the staining protocol. Once confirmed by the user for full ...

Discuss with the core director before preparing your lysates. View detailed protocols for lysate preparation. Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting ... The facility provides expert consultation for protocol development so that experiments are appropriately designed and ...

The Cell Model and Evaluation Core was established in 2007 and is currently organized on the basis of the well-established notion that CF morbidity and mortality can be mitigated by an improved ...