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New approach identifies reproducible volatile organic compounds for lung cancer diagnosis
with inconsistent results even in in vitro cancer cell culture analyses. To address this, the team proposed using a combination of three different culture media—RPMI 1640, DMEM, and Ham's F12 ...
Nature8d
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus
the remaining effluent after isolating ECs and fibroblasts was resuspended in 5 mL RPMI medium. After washing, the cells were resuspended in VSMC medium and disseminated into 250-mL cell culture ...
Silk sponges instead of animal testing: How a 3D cell culture system could advance cancer diagnostics
A consortium of Austrian research groups from the University of Vienna, MedUni Vienna and Technikum Wien, together with ...
Nature14y
The novel JAK inhibitor AZD1480 blocks STAT3 and FGFR3 signaling, resulting in suppression of human myeloma cell growth and survival
Human myeloma U266, RPMI 8226, MM1.S, IM-9 and NCI-H929 cell lines, as well as bone marrow (BM) stromal cells (HS-5), were obtained from the American Type Culture Collection (Manassas, VA ...