As part of the VAX-seq process, the complementary DNA library preparation introduced two flanking-type adaptors to the messenger RNA's 5' and 3' ends. To identify complete-length molecules of mRNA ...

The term "transcriptome" refers to the set of genes that are expressed or transcribed from genomic DNA in a particular cell or tissue. An understanding of the transcriptome translates into an ...

Thus, single-primer PCR amplifies the genomic library and produces double-stranded DNA fragments with complementary adaptors. In addition to the complementary tags, two independent tags can be ...