Genome editing with various CRISPR-Cas molecule complexes ... The latter requires a copyable template for repair to accurately rejoin the DNA at the cut site. The slow variant is called homology ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, including off-target cutting. Over the years, scientists have ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

CRISPR technology has revolutionised genetic engineering, offering unprecedented precision in DNA editing with vast implications for medicine, agriculture and conservation. Yet, its power demands ...

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

A team of researchers at Karolinska Institutet has developed a novel tool for genetic research. The study, published in ...

Suppressing chromosomal DNA repair ability increased the rate of ... Trisomic rescue via allele-specific multiple chromosome ...

Joung, an early pioneer of the gene-editing technology, was the first to show CRISPR could target and cut DNA inside an embryo — in zebrafish — back in 2013. Not long after, his group was ...

The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell. After that, the next step in CRISPR gene editing is usually to alter that piece of DNA. However ...

CRISPR places an entirely new kind of power into human hands. For the first time, scientists can quickly and precisely alter, delete, and rearrange the DNA of nearly any living organism ...