The isolated plasmid DNA yield was reproducible across the samples with an average yield of 65.3 ng/μL (± 7.7 ng/μL SD). The average 260/280 ratio was 2.0 (± 0.05 SD), indicative of good DNA ...

DNA Plasmid Properties. DNA plasmids have several unique properties that make them naturally unique. They are regarded as replicons containing the origin of replication. They also have ...

Processes yielding >500 mg plasmid DNA/L have previously been reported by Merck, Boehringer Ingelheim, and Nature Technology (NTC). Common features among these high-yield processes include the use ...

February 15, 2008 (Vol. 28, No. 4) Boehringer’s New cGMP Production System Employs Prudent Vector Design as a Backbone For manufacturing plasmid DNA, good manufacturing practice begins with ...

RepC and a replication origin are essential for plasmid replication, ... DnaA and oriC, the bacterial mode to initiate DNA replication. FEMS Microbiol. Rev. 26, 355–374 (2002).

Thus, considerable effort has been made for plasmid detection and monitoring by using methods like complete plasmid DNA sequencing. Methods for plasmid detection . There are several approaches for ...

DNA-based information is a new interdisciplinary field linking information technology and biotechnology. The field hopes to meet the enormous need for long-term data storage by using DNA as an ...

Scientists who have described in a new study the step-by-step details of a bacterial defense strategy see the mechanism as a ...

The CRISPR system successfully incorporated the plasmid DNA into the bacterial genome, while the "self" DNA was only rarely attacked. The team recorded some 38 million separate immunization events.

Plasmid DNA isolation is an important technique in molecular biology, ... DH5α E. coli cells were transformed with cloning vectors with different origins of replication ...