To further increase the detection rate of the IGH PCR, several attempts have been made to design ... that the primer binding will not easily be affected by extensive nucleotide deletion in the ...

In this video from Thermo Fisher Scientific, a few tips for better PCR primer design are presented. PCR, Polymerase Chain Reaction, is a common and vital part of genetic studies, biomedical research ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

2, mutation shown in yellow). Design inverse PCR primers. Primers should overlap each other by 15 bp at their 5′ ends and incorporate desired mutation (deletion, substitution or insertion).