Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...

The PCR mix contained 8 pmol of each reverse and FAM-M13(-21) primer and 2 pmol of the forward primer in a final 50 μl reaction volume (Perkin-Elmer Standard PCR reaction buffer, 0.2 mM dNTPs ...