This process, known as blastocyst complementation, involves injecting rat stem cells into mouse blastocysts—early-stage embryos lacking the ability to develop a pancreas due to genetic mutations.

Once a strain was found that lacked a modified base, the gene responsible was identified by complementation with plasmids containing single genes within the deleted region, or by constructing ...

The semester will cover strategies ranging from the classical (screens, selection, complementation, and conditional mutants) to the modern approaches enabled by the genomic revolution (genetic ...