When DNA sequencing first broke onto the scene in the 1970s, one method shone brighter than even Sanger sequencing. However, this method is now almost obsolete. What was it and what happened to it? In ...

About three decades ago, the Sanger and Maxam-Gilbert methods revolutionized the biomedical world. Sequencing of the 5,386-base bacteriophage PhiX174 in 1977 represented a memorable moment in science.

The potential of sequencing technologies was quickly recognized ... acids”, a mere 3 years after the development of Maxam–Gilbert sequencing and Sanger sequencing in 1977.

The sequencing of DNA molecules started with the development of chemical cleavage method of Maxam-Gilbert during 1970s followed by di-deoxy chain termination method. However, the whole genome ...