In the mid-1970s, two techniques for directly sequencing DNA were developed – the well-known and still used Sanger chain-termination method and a chemical sequencing method that has been almost ...

The potential of sequencing technologies was quickly recognized, with half of the 1980 Nobel Prize in Chemistry being awarded to Walter Gilbert and Frederick Sanger “for theircontributions ...

About three decades ago, the Sanger and Maxam-Gilbert methods revolutionized the biomedical world. Sequencing of the 5,386-base bacteriophage PhiX174 in 1977 represented a memorable moment in science.