Bacterial suspensions were delivered to the surface of Mueller-Hinton agar plates using a multipoint inoculator (MIT-60 P; Sakuma Seisakusho, Tokyo, Japan). The final inoculum was ~10 4 CFU ml −1 .

Edge makes Mueller Hinton and Thayer Martin Agar products. Mueller Hinton Agar is a microbiological growth medium that is commonly used for testing antibiotic susceptibility.