DPO primers are an essential component ... TOCE is a major advancement in high-multiplex real-time PCR capabilities, offering assay design that greatly expands the capabilities of conventional ...

Unlike other highly multiplexed PCR approaches, NuProbe's SADDLE approach uses computational primer design to minimize primer-dimer formation, and thus does not require enzymatic primers and ...

“Primer design is both an art and a science ... or amplify off-target sequences. “Multiplex PCR typically requires an additional oligo called a probe. To multiplex two or more assays, these ...

Some of the important design considerations for a primer used in multiplex PCR are primer length, melting temperature, primer specificity, and control of the formation of primer dimers.