The basic concept behind the extended depth of field is that a specially designed phase plate is inserted at the back aperture of the microscope objecive. The phase plate creates a uniquely shaped PSF ...

In light microscopy this is achieved by using Zernike's 90° phase plate which advances the phase of the scattered beams into an antiphase relation to the incident beam. This makes the scattering ...

and Lawrence Berkeley National Laboratory (Berkeley Lab), where the continuous laser phase plate for electron microscopy was invented and continues to be developed. Thermo Fisher will work ...