Plasmid Conjugation (IMAGE) Duke University. Caption. This movie depicts the basic process of antibiotic resistance spreading through plasmid conjugation, otherwise known as horizontal gene ...

In classical bacterial conjugation, one bacterium transfers genetic material to another through direct cell-to-cell contact. 3 This can either be through a natural process or via artificial means.

Conjugation is the process by which one bacterium transfers genetic material to another through direct contact. ... Typically, the genetic material is in the form of a plasmid, ...

Plasmid conjugation is usually measured by observing hudreds of bacterial colonies that take 16 hours to replicate. This laborious process was replaced by a method created by Bethke and Allison ...

In order for conjugation to start, an enzyme is needed -- a relaxase -- which fits onto a specific location on the plasmid. The relaxase has to recognise and bind to a region where the DNA ring ...

By measuring the rate of plasmid conjugation both with and without beta-lactamase antibiotics present, they showed that, except for one outlier, these antibiotics do not increase the rate of ...

More information: Logan Ott et al, Dietary zinc supplementation inhibits bacterial plasmid conjugation in vitro by regulating plasmid replication ( rep ) and transfer ( tra ) genes, Applied and ...

Plasmid delivery through conjugation between bacterial species has proven effective in creating novel antimicrobial agents, targeting specific genes to eliminate or suppress pathogens.

While conjugation has traditionally been associated with bacteria attaching to solid surfaces, the team investigated pLS20, a widespread conjugative plasmid in Bacilli species, ...

Ott, L., et al. (2024) Dietary zinc supplementation inhibits bacterial plasmid conjugation in vitro by regulating plasmid replication (rep) and transfer (tra) genes. Genetics and Molecular Biology ...