The EGFP-PC3.1 plasmid was prediluted in Opti-MEM (1 µg/100 µL). The transfection-complex was formed by pipetting the same amount of plasmid dilution into the dilution of transfection reagent ...

Credit: Technology Networks. A) Before transfection: Plasmid DNA: The quality of plasmid DNA is very important for efficient transfection. The gene of interest is usually cloned into an appropriate ...

This study shows that sinusoidal focused ultrasound can significantly enhance the transfection of plasmid DNA in the Dunning prostate tumor R3327-AT1 in vitro and in vivo after direct plasmid ...

Contamination with bacteria, yeast, virus, or mycoplasma will not only impair cellular health but also transfection efficiencies. The plasmid DNA concentration needs to be sufficient to obtain ...

Thermo Fisher Scientific has introduced the Applied Biosystems™ MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit to meet the ...

Plasmid DNA (pDNA) is an essential component of molecular biotechnology ... The AmMag™ Quatro provides scientists with an automated route to purify high-quality, transfection-grade plasmids.

Analytik Jena‘s CyBio FeliX Liquid Handler facilitates the automatic isolation of transfection-quality plasmid DNA by way of applying a protocol for Promega‘s Wizard MagneSil Tfx ™ custom ...

A novel cationic nanotechnology called jetOPTIMUS ® was created to increase the effectiveness of DNA transfection in adherent cells, including difficult-to-transfect cells. Transfection with ...

Researchers deliver transfected nucleic acids as oligonucleotides or in a viral or plasmid vector, which carries the genetic material into the host cells. Transfection can be stable or transient.