Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all. Recombinase-based methods allow scientists to move pieces of DNA from plasmid to ...

as the constructs carry the necessary antibiotic resistance selection markers and a unique homing endonuclease restriction site for linearization of the multigene plasmid. In this study ...

Well, you’ll go “ApE” over A Plasmid Editor ... as well an option to identify and display restriction enzyme sites within the DNA. Users can perform “virtual digests” whereby they ...

Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at specific sites and then ... you will carry out restriction digestion of the genomic clone, in order to cut ...

These restriction sites are often clustered in polylinker ... typically a virus or a plasmid that is used as a vehicle to carry a DNA sequence into a host cell as part of a molecular cloning ...

cut by specific restriction enzymes, have a unique location in that plasmid. These sites flank the cloning site in the plasmid. Many modified plasmids have a variety of unique restriction fragment ...

Because the enzymes will cut anywhere that sequence appears, researchers rely on finding a restriction enzyme whose cut site appears only once in the genome of their organism or plasmid ...