Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all. Recombinase-based methods allow scientists to move pieces of DNA from plasmid to ...

Prepare plasmid DNA using standard miniprep protocols ... Alternatively, the loss of the unique linker restriction sites can also be used as a diagnostic. Confirm that the GFP fusions are the ...

Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at specific sites and then ... you will carry out restriction digestion of the genomic clone, in order to cut ...

The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the T-DNA at a specific restriction site. Subsequently, foreign DNA, which has been cleaved by the same enzyme, ...