Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all. Recombinase-based methods allow scientists to move pieces of DNA from plasmid to ...

(a) HEK293 cells were transiently transfected with a mixture of five plasmids, each containing an expression cassette for a single fluorescent protein or (b) a single MultiLabel plasmid containing ...

“Using our technology, we can create highly active artificial restriction enzymes with virtually any sequence specificity and defined sticky ends of varying length,” said Huimin Zhao, Ph.,D ...

Well, you’ll go “ApE” over A Plasmid Editor, a program as simple in use as it is in name. ... as well an option to identify and display restriction enzyme sites within the DNA.

If you want to digest a restriction site that may be methylated, you can use a methylation-incompetent E. coli strain (JM11) to propagate your plasmid. These E. coli strains cannot methylate DNA so ...

These sites flank the cloning site in the plasmid. Many modified plasmids have a variety of unique restriction fragment sites, formed by unique combinations of restriction enzymes.

1. Prepare the restriction enzyme digests. You will perform two single digests using enzymes EcoRI and HindIII and one double digest using both enzymes. You will also be given some plasmid prepared by ...