His method relies on the occurrence of the primer-dimer artifacts in PCR reactions that compete with template DNA. The ratio of input DNA/primer-dimers will determine the amount of template DNA ...

PCR was developed in 1983 ... For example, if primers bind to each other, the dye will bind to these so-called primer dimers and generate a signal. In addition, SYBR Green I cannot be used ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...