However, choosing a suitable fluorescent probe, dye, or biosensor to visualize a specific living process can be daunting, given the countless molecules available. Researchers can simplify fluorescent ...

The 5' exonuclease activity of Taq polymerase triggers the release of a fluorescent dye upon hybridization of the probe with its complementary sequence, resulting in the cleavage of the dye.

The far-red emission of the dye facilitates multiplexing with GFP and other fluorescent probes. With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning ...

In addition, the probe has a label, which is typically a radioactive atom or a fluorescent dye. Thus, following hybridization, the probe permits the RNA molecule of interest to be detected from ...