Improved MATQ-seq workflow for bacterial single-cell RNA-seq. (A) Overview of bacterial scRNA-seq pipeline including major steps from cell culture to bioinformatic analysis. Changes from the ...

Researchers in Würzburg have refined MATQ-seq, a powerful bacterial single-cell RNA sequencing method that captures gene activity in 95% of cells. The protocol analyzes 300–600 genes per bacterium and ...

But existing tools cannot be used to apply single-cell RNA sequencing to examine all the molecular relationships between thousands of bacterial subspecies and the no-less impressive array of hosts. To ...

Additionally, as targeted sequencing of 16S rRNA is the gold standard for identifying different species of bacteria, this method can leverage established and easy-to-use protocols and analysis ...

High-throughput RNA sequencing (RNA-Seq) is a cutting-edge technology that uses deep sequencing techniques to analyze an RNA population converted into a library of complementary DNA (cDNA) fragments.

In this study, scientists mapped host–bacterial cellular, spatial, and molecular interactions within the TME using single-cell RNA sequencing (scRNA-seq) and in situ spatial-profiling technologies.

Years of experiments and databases filled with RNA-seq results belie the simple reality that, until fairly recently, it was impossible to analyze RNA directly. The RNA-seq studies performed with ...

This exciting new technology, which the authors call M3-seq (short for “massively parallel, multiplexed, microbial sequencing”), is not limited to use on single species of bacteria.

Scientists at McMaster University have identified a toxin used by bacteria to kill other bacteria through a never-before-seen mechanism. The toxin is the first found to directly target RNA ...

Universal RNA barcoding system for tracking gene transfer in bacteria New method has far-reaching applications in medicine, biotechnology and environmental science Date: March 18, 2025 Source ...