With a modified polymerase and optimized oligonucleotide chemistry, Helicos' single-molecule sequencer takes on RNA. RNA sequencing (RNA-seq) is actually a misnomer for the increasingly popular ...

Conventional targeted RNA-seq methods often require labor-intensive pre-sequencing enrichment steps, which can compromise ...

They ruled out using direct capture methods to prepare samples for RNA-seq, as chloroplast mRNAs are not typically polyadenylated. They had also previously encountered issues with probe-based rRNA ...

Spatial transcriptomics is a cutting-edge technique that characterizes gene expression within sections of tissue, such as ...

Technology Networks spoke to the most productive researcher in spatial transcriptomics, Professor Joakim Lundeberg, about the ...

In this interview, we gain a behind the scenes look at Morgan’s recently published paper in BioTechniques, ‘RNA Extraction and RNA-Sequencing Method for Transcriptomic Analysis of Mycobacterium ...

The immune system responds to an infection by producing antibodies that recognize and bind to the cell surface of the pathogen, thus marking it as an intruder and triggering an immune response. For ...

In the microscopic world of bacteria, gene transfer is a powerful mechanism that can alter cellular function, drive antibiotic resistance and even shape entire ecosystems.

2017 Cell; Li et al 2020 Current Biology). Recently, we developed a single-nucleus RNA-seq method in flies (McLaughlin, …, Li, 2021 eLife) and applied it to the Fly Cell Atlas (FCA) project, a large ...

The lab focus on pancreatic cancer in particular. Development of Quantitative Single-cell RNA-seq methods for Studying Tumorigenesis and Aging processes The quantification of transcriptional variation ...

Single-cell transcriptomics has revolutionized the study of cellular diversity and function by enabling gene expression ...