PolyA-RNA-seq: Capture of coding RNAs. Requires intact RNA. Full-length transcripts are captured. Recommended read depth is 15-30 million reads/sample although deeper sequencing may be required in ...

PolyA-miner: accurate assessment of differential alternative poly-adenylation from 3′Seq data using vector projections and non-negative matrix factorization. Nucleic Acids Research , 2020; 48 ...

Meet PolyA-miner, a new computational tool that enables scientists to evaluate the contribution of alternative polyadenylation to gene regulation in health and disease.

When the machinery of the cell detects a polyA signal in the RNA, it automatically makes a cut and defines the cut point as the end of the RNA. “In our system, we use the added polyA signal, not ...

Two-Step RNA-Seq. Used with either isolated nuclei (pictured) or synaptosomes, MATQ-Drop starts with internal primers to reverse-transcribe mature, immature, and noncoding RNA; in the second step, ...

I n Chinese mythology, a cunning fox with nine tails symbolizes peace and prosperity. Xiao Wang, a chemical biologist at the Massachusetts Institute of Technology, took a bit of scientific inspiration ...

A new study reports the use of a modified deoxyribonucleic acid (DNA) oligonucleotide that has a 3’ polyadenine (polyA) tail to bind to the 5’-polyU tract in the mouse coronavirus (MHV-A59).

RNA-Seq from 2.8 Million Cells Yields New Clues About Alzheimer's 21 Sep 2022; Paper Citations. Welch JD, Kozareva V, Ferreira A, Vanderburg C, Martin C, Macosko EZ. Single-Cell Multi-omic Integration ...

More information: Hari Krishna Yalamanchili et al, PolyA-miner: accurate assessment of differential alternative poly-adenylation from 3′Seq data using vector projections and non-negative matrix ...