DNA microarrays have been used to study the expression of thousands of genes at the same time in a variety of cells and tissues1,2,3. The methods most commonly used to label probes for microarray ...

A limiting aspect of microarray experiments is the efficient production of labelled cDNA probes from small amounts of starting material (typically over 20 μg of total RNA is required) . In the July ...

Compared to random-primer probes, the GDP probes were more sensitive and more specific, especially when mammalian RNA samples were spiked with mycobacterial RNA.

Multiple cross displacement amplification (MCDA) method uses random primers and DNA polymerase to amplify the circular DNA template. The DNA can be amplified over 10,000-fold within a few hours.