Reversed-phase column (Brownlee RP-300 cartridge; 100 mm length × 2.1 mm inner diameter, octylsilica 300 Å pore size, 7 μm diameter of the particle; PerkinElmer) Size-exclusion columns ...
Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).
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