In Sanger sequencing, the DNA to be sequenced serves ... and T reactions are individually loaded into four lanes of a single gel and separated using gel electrophoresis, a method that separates ...

Sanger sequencing (left) sequences 500-700 bases per reaction with 16 reactions per gel. Massively parallel DNA sequencing (center) sequences 100-5,000 bases per reaction with 10 thousand to 10 ...

“There is significant debate within the diagnostics community regarding the necessity of confirming NGS variant calls by Sanger sequencing, considering that numerous laboratories report having 100% ...

In addition, whereas traditional, gel-based Sanger sequencing and early machines could only generate 250 to 500 base pairs of DNA sequence per reaction, 750 to 1,000 base pairs of sequence can now ...