In Sanger sequencing, the DNA to be sequenced serves ... and T reactions are individually loaded into four lanes of a single gel and separated using gel electrophoresis, a method that separates ...

Sanger sequencing (left) sequences 500-700 bases per reaction with 16 reactions per gel. Massively parallel DNA sequencing (center) sequences 100-5,000 bases per reaction with 10 thousand to 10 ...

In more than 90% of failed sequencing reactions the cause is insufficient DNA quality ... If you have unpurified products, include the picture of the agarose gel electrophoresis with loading amounts.

In addition, whereas traditional, gel-based Sanger sequencing and early machines could only generate 250 to 500 base pairs of DNA sequence per reaction, 750 to 1,000 base pairs of sequence can now ...