To run a Sanger sequencing reaction you need template DNA, a single stranded DNA primer to get the reaction started, DNA polymerase, and nucleotides (G's, C's, A's, and T's). However, some of the ...

Due to the use of dideoxynucleotides in the reactions, Sanger sequencing is also called "dideoxy" sequencing.

The Broad had recently eliminated a number of its Sanger machines ... to shrink down the amount of DNA that's required for a sequencing reaction, in at least one case dropping it to a single ...

In Sanger sequencing, chain-terminating nucleoside bases are incorporated into the DNA replication reaction. This results in a series of DNA chains truncated at each base pair in the sequence.

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

MADISON, Wis.--(BUSINESS WIRE)--Research labs performing Sanger sequencing can now use Promega chemistry on any capillary electrophoresis (CE) platform. The ProDye™ Terminator Sequencing System ...

As a starting material we use purified PCR products mixed with sequencing primer or ready made sequencing/fragment analysis reactions. Maximum read length ... to iLab and create a service request for ...