For second-strand synthesis, 25 μl of purified cDNA was added to 65 μl of Klenow master mix (46 μl of water, 10 μl of 10× NEBuffer 2, 5 μl of 10 mM dNTPs, 4 μl of 5 units μl −1 exo − ...

Second-strand cDNA synthesis: mRNA fragments as primers. Methods Enzymol. 152 , 330–335 (1987). Article CAS Google Scholar ...

Unbiased profiling of RT-associated RNA and DNA ligands in DRT2-expressing cells revealed that reverse transcription generates concatenated cDNA repeats through programmed template jumping on the ...

The kit contains all required components for first-strand cDNA synthesis except RNA template and gene-specific primers. Available in 25 or 100 reactions, the kit is compatible with downstream 2 ...