mRNA-FISH and single-cell RT-qPCR are suitable for the analysis of known genes of interest. But for biomarker discovery from a large gene pool, says Bio-Rad, RNA-seq is preferable.

While physical activity is known to slow cognitive decline in Alzheimer’s disease, many patients are unable to exercise due ...

Single-nucleus RNA sequencing (sNuc-seq) ... To address this challenge, we 3 and others 4,5,6 developed methods to analyze RNA in single nuclei from fresh, frozen, or lightly fixed tissues.

Single-cell sequencing is revolutionizing oncology. The ability to distinguish every cell and provide a far greater depth of analysis about cell-to-cell relationships is an increasingly powerful tool ...

For years, researchers have seen a connection between exercise and the progression of cognitive disorders such as Alzheimer's ...

Finally, the authors compared MATQ-Drop to a commercial platform for single-nuclei RNA-Seq, the 10x Genomics Chromium. When both methods were applied to nuclei isolated from mouse hippocampus, ...

A new study has shed light on how physical activity helps rewire and protect the brain from Alzheimer's disease on a cellular ...

RNA extraction from pancreatic tissue is difficult and prior single-cell RNA-seq efforts have been limited by suboptimal dissociation/RNA quality and poor ... Combined treatment-naïve (n=13) and ...

Scientists explore how single-cell RNA sequencing transforms plant biology, with new methods yielding richer insights and broader applications. Advanced Search Home ...

Researchers performed single-nucleus RNA sequencing (snRNA-seq) and spatial transcriptomic profiling of livers from deceased coronavirus disease 2019 (COVID-19) patients.

In this interview, News Medical talks to DeNovix about the best way to prepare nuclei samples before single cell sequencing workflows and optimize nuclei extraction.