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Reengineered streptavidin mutants bind biotin-4-fluorescein with extremely tight yet reversible binding affinity. The terrifically tight noncovalent interaction between biotin and streptavidin has ...
The dual biotin group is essential to keep the oligonucleotide attached to the streptavidin-coated beads during thermal cycling 4,5. Primers 5 and 6 are unmodified oligonucleotides with the Tag1 ...
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