Then, to perform PCR, the DNA template that contains the target is added to a tube that contains primers, free nucleotides, and an enzyme called DNA polymerase, and the mixture is placed in a PCR ...

The PCR cycling reactions are carried out in an automated thermocycler, which is capable of heating and cooling the reaction tubes in a short span of time. PCR-Machines are classified depending on ...

1| In a sterile 0.5-ml microcentrifuge tube or amplification tube (on ice), mix the following reagents for the first amplification reaction: 2| Place the tubes in a thermocycler and amplify the ...