A protocol for 5′ end cDNA amplification by classic RACE is presented here. 3′ end cDNA amplification can also be performed using a classic RACE protocol as described in a separate protocol 16.

We prepared cDNA from a single HeLa cell using the MessageBOOSTER kit and the cDNA-from-cells procedure (which does not include an RNA amplification step), along with the no-RT control.

CLONTECH's SMART RACE cDNA amplification process diagram On your mark, get set, go! And the RACE is on to obtain that potentially elusive full-length cDNA for characterizing the best gene ever. In the ...

The kit contains all required components for first-strand cDNA synthesis except RNA template and gene-specific primers. Available in 25 or 100 reactions, the kit is compatible with downstream 2 ...

TAS-Seq is a robust and sensitive amplification method for bead-based scRNA-seq. Communications Biology , 2022; 5 (1) DOI: 10.1038/s42003-022-03536-0 Cite This Page : ...

NuGEN Technologies, Inc. has announced the launch of the WT-Ovation™ Pico RNA Amplification System and the FL-Ovation™ cDNA Biotin Module V2. The latest amplification product in NuGEN's Ovation™ ...

The new method, terminator-assisted solid-phase cDNA amplification and sequencing (TAS-Seq), uses simple materials and equipment to provide higher-precision scRNA-seq data than current, ...

NuGEN Technologies Inc. has announced the launch of the WT-Ovation™ RNA Amplification System. WT-Ovation™ utilizes an amplification approach that is based on NuGEN's proprietary Ribo-SPIA™ technology, ...

Combination will be provided for use on Agilent’s DNA microarrays for gene-expression analysis. NuGEN Technologies plans to incorporate Kreatech Biotechnology’s FL-Ovation™ cDNA Fluorescent ...