consisting of about 18 600 fully verified cDNA clones, and a collection of full-length cDNAs (MGC collection) consisting of about 29 000 clones. The ORF library clones are available in Gateway-based ...
1). Nucleotide sequencing of cDNA clones, and rescreening of libraries as necessary, resulted in full-length cDNA sequences for 23 novel germ-cell–specific genes (Table 2). Figure 1: Expression ...
Repeat the dilution step and centrifuge again. Transfer the retentates containing cDNA to fresh 0.5-ml microcentrifuge tubes, and reduce the volumes to ∼10 μl in a rotary vacuum evaporator.