The expenses associated with library preparation begin to dominate the cost of the entire NGS workflow as the cost of sequencing falls. A number of institutions are adopting the trend of reaction ...

and many companies will offer a library preparation kit. You will need to consider reverse transcription of RNA and cDNA synthesis, PCR amplification and whether or not to preserve strandedness. The ...

These workflows often start with an RNA library preparation kit to perform cDNA synthesis and generate sequencing libraries from RNA. The libraries are then combined with biotin-labeled ...

The cDNA is then fragmented, and adapters are added to each ... These include: The impact of sample pooling – pooling samples prior to library preparation (without the use of barcoding) can reduce ...

SEQuoia Complete Stranded RNA Library Prep Kit is a high-performance RUO RNA-Seq kit that ... and liquid biopsy specimens, resulting in cDNA libraries suitable for strand-specific NGS in less than 4 ...

Using the new system, sample preparation of 96 CAGE cDNA libraries, which would normally take a manual operator 42 days, is done in only 8 days. The researchers further demonstrated, through a ...

Using the new system, sample preparation of 96 CAGE cDNA libraries, which would normally take a manual operator 42 days, is done in only 8 days. The researchers further demonstrated, through a ...