In this article, we will consider how qPCR works, experimental requirements, data analysis and applications of the technique.

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

led to the discovery that PCR amplification can be monitored in real time ... and it shows superior performance when used in qPCR and for high resolution melt curve analysis. This makes it a promising ...

RT-PCR and qPCR: Learning the differences between RT-PCR (used for ... template preparation, and reaction components. Amplification Curves: Interpreting amplification curves to monitor the progress of ...

5. Like all forms of PCR, qPCR and RT-qPCR requires optimization to ensure sufficient and specific amplification. You may need to optimize PCR parameters such as magnesium concentration ...

Falling cost of nucleic acid detection, quantification, and amplification techniques will additionally encourage adoption of qPCR and dPCR instrumentation in research and diagnosis, particularly ...

As mentioned previously, melt curve analysis can be used to check the identity of qPCR products, particularly helpful for dye-based amplification. Positive control wells (in a well-designed assay) ...