we first investigated the impact of functional driver gene knockout on cancer cell viability in the DLBCL cell line OCI-LY3 in a non-mutation-specific manner. To ensure the reliability and efficacy of ...

Poseida Therapeutics’ gene therapy for hereditary angioedema (HAE) reduced kallikrein protein activity in a mouse model of the ... more reliably than the CRISPR-Cas9 approach, according to ...

Mice constitute the most widely used model since their retinal structure is similar to humans and they can easily provide knock-out/-down models ... Comparison of CRISPR/Cas9 nucleases, base editors, ...

In an extraordinary 6,000-word guest editorial in the October 2024 issue of The CRISPR Journal (a sister journal of GEN, published by Mary Ann Liebert, Inc.), Fyodor Urnov, PhD, laid out the ...

It allows the evaluation of the quality of gene editing experiments using targeted next generation sequencing (NGS) data (`targeted`) as well as the discovery of important genes from knock-out or ...

and all these fragments overlap only marginally with the mouse region. The CRISPR/Cas9 experiment to generate our knockout line might have generated potential off-target mutations. In order to rule ...

Ultimately, this could help with finding better ways to fight this deadly disease. Here we describe a CRISPR/Cas9-mediated gene knockout functional screening method in which lentiviral genomes ...