CAR T-cell killing assay. All steps of the killing assay were conducted in an E-Plate VIEW microplate (Agilent Technologies, part number 00300601030).

These CAR-T cells were then extensively characterized with pertinent analytical assays. In particular, PureQuant Methylation assay was used to measure percent of CD4/CD8 cells and also detect ...

The Agilent NovoCyte Quanteon flow cytometer and xCELLigence Real-Time Cell Analysis platform were used to develop a series of in vitro preclinical CAR T-cell safety and efficacy assays.

CAR-T-cell therapy has shown remarkable promise in addressing unmet medical needs in people with cancer. Continued progress in this field will depend, in part, on the advancement of frameworks for ...

Researchers were able to precisely quantify the number of CAR T cells in circulation and distinguish patients who responded. Skip to navigation Skip to main content. Menu Close.

Explore how effectively monitoring apoptosis and CAR expressions can be achieved by developing image-based T-cell assays. ... CAR-T cell viability results with premixed duo fluorescent AO/PI dyes.

Granzyme B assay in picodroplets with co-encapsulated CAR-T and target cells (bottom) or control cells (top). Fluorescence micrographs of picodroplets after 2, 4, and 24 h (left to right). Scale ...

Excitingly, these finding support that “this assay can be used in ongoing translational research studies to investigate CAR-T cell and TCR-T cell trafficking,” commented the authors. “Insight into ...

TruCytes Potency cell mimics offer a customizable, scalable, and efficient alternative to cell line target cells. This helps CAR-T developers accelerate assay validation and lot release, while ...

Chimeric antigen receptor (CAR) T cells have been strikingly successful in the treatment of hematologic cancers 1 but have not been effective against solid tumors thus far. 2 A recent report by ...