This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...

A scaleable method for the liquid-phase separation of plasmid DNA from RNA. In the course of experiments on the potential of nucleic acid compaction for enhancement of chromatographic adsorption ...

During this week you will carry out restriction digestion of the genomic clone, in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by ...

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes.