Before PCR, the process of DNA sequencing was very lengthy and quite expensive. How did the discovery of the Taq polymerase result in this important research technique? To amplify a DNA fragment ...

Eukaryotic RNA polymerases use a number of essential cofactors (collectively ... Once transcription is initiated, the DNA double helix unwinds and RNA polymerase reads the template strand, adding ...

The team focused on T7 DNA polymerase’s thioredoxin binding domain (TBD), which has a structure similar to that of most thermostable polymerases, including Taq. Thioredoxin, which is ubiquitous in ...