The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

In the early days of rAAV, cultured cells would first be infected with adenovirus, after which they would be transfected with two DNA plasmids ... Furthermore, the transfection procedures used ...